Picture 17

This picture shows Escherichia coli (in red) and Salmonella typhi (in yellow) cultured in MacConkey's agar. E. coli ferments lactose and therefore appears red in the neutral red indicator found in the culture medium, whereas S. typhi does not and remains yellow.

Escherichia coli

Morphology: Gram negative cocci that are facultative anaerobes, ferment lactose, are commensals in human gastrointestinal tract

Route of transmission: Food borne

Investigations: Gram stain, organism cultured on MacConkey's agar

Diseases:

Urinary tract infections

Neonatal meningitis

Enteric infections (Traveler's diarrhoea)

Treatment:

Penicillin

Cefotaxime

 

Salmonella typhi

Morphology: Gram negative cocci that do not ferment lactose, do not produce gas like other species of Salmonella but produce H2S in small quantities

Route of transmission: Food borne

Investigations: Gram stain, organism cultured on MacConkey's agar and Triple Sugar Iron (TSI) medium

Diseases:

Gastroenteritis, from diarrhea to dysentery

Enteric fever (Typhoid fever)

Septicemia

Treatment:

Gastroenteritis is a self-limiting disease, so no treatment is needed

Ceftriaxone and ciprofloxacin are prescribed for enteric fevers and septicemia

 

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