FRONTIERS IN BIOSCIENCE; PROTOCOLS
	[Molecular biology protocols] [Machines, Media and Reagents] [Purification] [Manipulation] [Transformation/Library Preparation] [Blotting] [Sequencing] [Oligonucleotides] [PCR] [Protein Electrophoresis]
	C elegans protocols Molecular biology protocols (1) Molecular biology protocols (2) Other biology protocols Machines, Media and Reagents ABI 373 Loading Guide ABI 377 Loading Guide Media recipes Molecular reagent list Plate Pouring DNA Purification [Plasmids] [Phage(mids)] [Cosmids] [Diatomaceous Earth] [Special cases] [Reprecipitation] Plasmids Plasmid mini-prep I Plasmid mini-prep II Phages/Phagemids Large scale M13 prep M13 0.5 Thermo MAX prep M13 PEG prep I M13 PEG prep II M13 RF prep large scale M13 Thermo MAX prep M13 helper phage Single stranded template from pBluescript phagemid Cosmids Cosmid prep Codes for cosmid vectors Cosmid 96 well mini-prep Cosmid finger printing DNA purification (plasmids, cosmids and p1) using Diatomaceous Earth Introduction Maxi-preps and all media, solutions Midi-preps Mini-preps Special cases DNA isolation from low melting-temperature agarose DNA purification, bacterial-genomic, triton extraction method DNA purification, bacterial-genomic via Qiagen columns Low copy plasmid purification using Qiagen Midi columns Reprecipitation DNA/RNA precipitation DNA Manipulation PUC pooling Restriction Digest T7 gene 6 exo TA Cloning Vector preparation Vectors DNA Transformation/Library Preparation Transformation-Competent E. coli preparation CaCl Transformation Electrotransformation Inoue "ultra-competent" method JS5 Transformation Rubidium chloride method Cosmid and phagemid preparation Cosmid packaging protocol Preparation of M13 sub-libraries Southern/Northern Blotting Southern blot-chemiluminescent detection (BM "Genius" system) DNA Sequencing AGTC 96 well mini-prep Sequencing gel solutions Sequencing gels: wedge gel performance without pouring wedge gels Sequenase 1 dye-termination Sequenase II ALF primers Sequenase V2.0 licor Terminator reaction cleanup Oligonucleotides Oligonucleotide Visualization Oligo nucleotides PCR and Related Methods Core sample PCR: a method to re-PCR unique bands from products of mixed size Cloning PCR-Generated DNA Fragments, submitted by Hugh Griffin, 7/7/95 Site-directed Mutagenesis using PCR, submitted by Hugh Griffin, 7/25/95 Reverse Transcriptase PCR, submitted by Jim Hutchins 8/25/95 Error Rates for Thermal Resistant DNA Polymerases Linear amplification sequencing I Linear amplification sequencing II Linear amplification sequencing III Linear amplification sequencing IV Licor PCR of M13 inserts ss PCR magnetic beads Protein Electrophoresis Tricine-polyacrylamide gels: for high resolution of small proteins SDS-polyacrylamide gels: standard Laemmli protocol