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- C elegans protocols
- Molecular biology protocols (1)
- Molecular biology protocols (2)
- Other biology protocols
Machines, Media and Reagents
- ABI 373 Loading Guide
- ABI 377 Loading Guide
- Media recipes
- Molecular reagent list
- Plate Pouring
DNA Purification
[Plasmids]
[Phage(mids)]
[Cosmids]
[Diatomaceous Earth]
[Special cases]
[Reprecipitation]
- Plasmids
- Plasmid mini-prep I
- Plasmid mini-prep II
- Phages/Phagemids
- Large scale M13 prep
- M13 0.5 Thermo MAX prep
- M13 PEG prep I
- M13 PEG prep II
- M13 RF prep large scale
- M13 Thermo MAX prep
- M13 helper phage
- Single stranded template from pBluescript phagemid
- Cosmids
- Cosmid prep
- Codes for cosmid vectors
- Cosmid 96 well mini-prep
- Cosmid finger printing
- DNA purification (plasmids, cosmids and p1) using Diatomaceous Earth
- Introduction
- Maxi-preps and all media, solutions
- Midi-preps
- Mini-preps
- Special cases
- DNA isolation from low melting-temperature agarose
- DNA purification, bacterial-genomic, triton extraction method
- DNA purification, bacterial-genomic via Qiagen columns
- Low copy plasmid purification using Qiagen Midi columns
- Reprecipitation
- DNA/RNA precipitation
DNA Manipulation
- PUC pooling
- Restriction Digest
- T7 gene 6 exo
- TA Cloning
- Vector preparation
- Vectors
DNA Transformation/Library Preparation
- Transformation-Competent E. coli preparation
- CaCl Transformation
- Electrotransformation
- Inoue "ultra-competent" method
- JS5 Transformation
- Rubidium chloride method
- Cosmid and phagemid preparation
- Cosmid packaging protocol
- Preparation of M13 sub-libraries
Southern/Northern Blotting
- Southern blot-chemiluminescent detection (BM "Genius" system)
DNA Sequencing
- AGTC 96 well mini-prep
- Sequencing gel solutions
- Sequencing gels: wedge gel performance without pouring wedge gels
- Sequenase 1 dye-termination
- Sequenase II ALF primers
- Sequenase V2.0 licor
- Terminator reaction cleanup
Oligonucleotides
- Oligonucleotide Visualization
- Oligo nucleotides
PCR and Related Methods
- Core sample PCR: a method to re-PCR unique bands from products of mixed size
- Cloning PCR-Generated DNA Fragments,
submitted by Hugh Griffin, 7/7/95
- Site-directed Mutagenesis using PCR,
submitted by Hugh Griffin, 7/25/95
- Reverse Transcriptase PCR, submitted by Jim Hutchins 8/25/95
- Error Rates for Thermal Resistant DNA Polymerases
- Linear amplification sequencing I
- Linear amplification sequencing II
- Linear amplification sequencing III
- Linear amplification sequencing IV Licor
- PCR of M13 inserts
- ss PCR magnetic beads
Protein Electrophoresis
- Tricine-polyacrylamide gels: for high resolution of small proteins
- SDS-polyacrylamide gels: standard Laemmli protocol
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